This web page was produced as an assignment for Genetics 564, an undergraduate course at the University of Wisconsin-Madison.
Protein Interaction Networks
What are Protein Interaction Networks?:
Protein-protein interaction maps provide valuable information regarding the functional organization of the proteome. These interactions are crucial to a proteins function [1]. There are many different experiments that can be conducted to determine what proteins are interacting with each other such as a Yeast Two Hybrid, Coimmunoprecipitation or Tap Tagging. Results from these types of experiments have been compiled and there are now databases that can be used to obtain information regarding known protein interactions, for example STRING.
Protein-protein interaction maps provide valuable information regarding the functional organization of the proteome. These interactions are crucial to a proteins function [1]. There are many different experiments that can be conducted to determine what proteins are interacting with each other such as a Yeast Two Hybrid, Coimmunoprecipitation or Tap Tagging. Results from these types of experiments have been compiled and there are now databases that can be used to obtain information regarding known protein interactions, for example STRING.
CNGA3 Protein Interaction Network?:
I used string to gather information on CNGA3's protein interaction network. The results obtained are showed and analyzed below.
I used string to gather information on CNGA3's protein interaction network. The results obtained are showed and analyzed below.
Discussion:
CNGA3 has a relatively small amount of protein interaction partners. Concurrent with what I would expect, all but one, EMILIN1, of the binding partners have functions that are directly related to vision. Many of the binding partners that SMART found are other subunits of the channel that CNGA3 helps make up. These proteins are boxed in blue. The protein binding partner boxed in red is EMILIN1. This protein has cell adhesion properties and has been sited in literature as a protein that could be studied to gain more insight into the role that CNGA3 has in vision. For more information regarding EMILIN1 look at the future directions page.
CNGA3 has a relatively small amount of protein interaction partners. Concurrent with what I would expect, all but one, EMILIN1, of the binding partners have functions that are directly related to vision. Many of the binding partners that SMART found are other subunits of the channel that CNGA3 helps make up. These proteins are boxed in blue. The protein binding partner boxed in red is EMILIN1. This protein has cell adhesion properties and has been sited in literature as a protein that could be studied to gain more insight into the role that CNGA3 has in vision. For more information regarding EMILIN1 look at the future directions page.
Resources:
[1] Stelzl, U. et al. (2005). A Human Protein-Protein Interaction Network: A Resource for Annotating the Proteome. Cell. 122: 957-968. doi: 10.1016/j.cell.2005.08.029
[1] Stelzl, U. et al. (2005). A Human Protein-Protein Interaction Network: A Resource for Annotating the Proteome. Cell. 122: 957-968. doi: 10.1016/j.cell.2005.08.029